hcPRP Methodology

 

The hcPRP Methodology was invented by Dr. Astley Smith to facilitate the production, activation and clinical applications in Medicine, Dentistry and Cosmetics. The mastery of the methodology involves extensive hands-on involvement in order to adequately reap the benefits. It is highly recommended that those who wish to use the methodology attend one of the monthly seminars presented by Global Autografting Institute. You can register online at “Register Now”.

 

 

Dr. Smith’s Medical Procedure for hcPRP9x-90x

Injection Autografts and Cartilage/Bone Autografts

 

Production and Activation of hcPRP

(One copy with Centrifuge)

 

BEFORE STARTING PRODUCTION USE A 1ml SYRINGE FILLED WITH WATER AND ACCURATELY PLACE MARKERS ON THE SIDE OF THE RED TOP TUBE TO INDICATE 1ml, 1.5ml, 2ml, 3ml, 4ml AND 6ml. USE THIS RED TOP TUBE AS A MEASUREMENT GUIDE FOR THE VOLUMES LEFT BEHIND AFTER PPP HAS BEEN REMOVED.

 

 

HAVE THE FOLLOWING LABELS AVAILABLE: SERUM, PLASMA, CaCL2, hcPRP AND PPP TO PLACE ON APPROPRIATE TEST TUBES AND NEEDLES TO PREVENT CONTAMINATION.

1.             Collect six 10ml vacutainer tubes (yellow top) of citrated blood (with anticoagulant)

                and 2 red top tubes of whole blood without anticoagulant.

 

2.             Set the two red top tubes of whole blood aside to clot for 15-20 minutes. 

 

3.             Place the six yellow top tubes of citrated blood into the centrifuge and spin at 1000

                rpm (120g) for 10 minutes. 

 

4.             Remove the tubes from the centrifuge one at a time, then using the 2½ inch needle,

                pierce the centre of the rubber stopper or remove the rubber stopper and aspirate the

                plasma from above the red blood cell layer aspirating from the top down and pool the

                plasma from 3 separate yellow top tubes into one red top tube labeled hcPRP.

                Similarly pool the plasma from the other 3 yellow top tubes into a second red top tube

                labeled hcPRP. Immediately discard all six tubes with the red blood cells left behind.

 

5.             Place the two red top tubes with plasma and labeled hcPRP along with the two red top

                tubes with clotted whole blood into the centrifuge and spin at 3000rpm for 10

                minutes.

 

6.             Using a second 2½ inch needle pierce the rubber stopper or remove the rubber stopper

                and aspirate the serum from the 2 tubes of clotted whole blood leaving behind the

                fibrin clot. Store the serum in the stoppered test tube labeled serum for platelet

                activation later.

 

7.             Using the first 2½ inch needle pierce the rubber stopper or remove the rubber stopper

                and aspirate platelet poor plasma (PPP) from the two remaining tubes, 

                leaving behind 2.5ml for 9x baseline, 2ml for 11x baseline, 1.5ml for

                15x baseline and 1ml for 23x baseline respectively. (Each with a platelet button. 

                Store PPP for use later.

 

 

8.             Pierce the rubber stopper with a 4 inch needle or remove the rubber stopper and use

                the 3 inch or 4 inch needle to suspend the platelet button in each of the two tubes by

                aspiration and ejection three times, and then pool into one tube for a total of 

                5mls for 9x baseline, 4mls for 11x baseline, 3mls for 15x baseline and 2ml for

                23x baseline. Stop at this point and proceed to activate hcPRP as outlined below. 

 

9.             If you require 30x baseline or 45x baseline repeat steps #7 and #8 but leave behind 2ml

                with a suspended platelet button. Then combine the two tubes to get 4ml of hcPRP.  

                Balance the hcPRP tube then proceed to the third spin at 3000rpm for 5 minutes, then

                remove platelet poor plasma leaving behind 1.5ml for 30x baseline and 1ml for 45x      

                baseline. Suspend platelet button as previously mentioned.  Stop at this point and proceed

                to activate hcPRP as outlined below.

 

10.           For arthroscopic and open conventional surgeries the two tubes of whole blood should

                be centrifuged first at 3000rpm for 5 minutes.  This will produce larger fibrin clots

                and the serum will activate platelets faster.

 

Guidelines for Activation of hcPRP and its Clinical Applications

 

For Injection Grafts

Added for Cartilage and Bone Grafts

Volume of

hcPRP

Volume of

CaCl2

Volume of

Serum

 

Total Volume

Collagen Sponge

(MRCS)

Fibrin Clot (MFC)

 

Bone

0.5ml

0.1ml

0.25ml

0.85ml

0.25 sheet

As needed

As needed

1.0ml

0.2ml

0.50ml

1.70ml

0.5 sheet

As needed

As needed

1.5ml

0.3ml

0.75ml

2.55ml

0.75 sheet

As needed

As needed

2.0ml

0.4ml

1.0ml

3.4ml

1.0 sheet

As needed

As needed

3.0ml

0.6ml

1.5ml

5.1ml

1.5 sheets

As needed

As needed

4.0ml

0.8ml

2.0ml

6.8ml

2.0 sheets

As needed

As needed

5.0ml

1.0ml

2.5ml

8.5ml

2.5 sheets

As needed

As needed

 

 

 

**The Same Ratios as above are used for the activation of Platelet Poor Plasma (PPP)**

*When collagen and/or bone are added the total volume will increase accordingly*

NO OTHER COMPONENT IS TO BE ADDED TO THE hcPRP

Dr. Astley E. Smith e-mail: globalautografting@telus.net, Phone: (604) 818-8767

© Global Autografting Inc. www.globalautografting.com    DO NOT COPY

Patent Pending